Reporter

Part:BBa_K3026015

Designed by: Pascale Lemieux   Group: iGEM19_ULaval   (2019-10-16)


Toehold measles virus MG912594.1

ToeHold switch targeting mRNA sequence of the MG912594.1 gene from norovirus genotype H1. This part is flanked by Gibson homology sites for plasmid pBluescript for insertion in cloning site. This part, when expressed in a cell or media containing T7 polymerase, will produce a ToeHold switch with a recognition sequence in the mRNA sequence of the MG912594.1 gene. When this ToeHold is in presence of this mRNA sequence, the sfGFP will be produced. This system allows for detection of RNA fragments or plasmids containing this cassette. For in vivo expression, use strain BL21 DE3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 167


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